Catalase Lab

Materials

• timer
• filter paper
• 100 mL graduated cylinder
• water bath
• 10 mL graduated cylinder
• cork/funnel with tube
• hydrogen peroxide (3% solution)
• liver 
• Erlenmeyer Flask

Procedure

1. The liver was ground up and mixed with the number of filter discs. 
2. One disc was taken placed in an Erlenmeyer flask.The opening of the flask was covered with the cork/funnel attached to a tube. The other end of the tube was placed into the water bath container.
3. The water bath was setup and the 100 mL graduated cylinder was filled to the rim with water and was flipped into the water bath. The opening of the graduated cylinder covered the tube.
4. A 10 mL graduated cylinder to measure 5.0 mL of hydrogen peroxide. 
5. The peroxide was put into the flask and the hole in the funnel was blocked.
6. Swirl/shake the flask until the gas stops displacing the water. 
7. Record the amount of gas produced
8. Discard the products down the sink and wash out the flask.
9. Repeat steps 2-8 for multiple trials with a variable number of filter discs

Qualitative Observations

Before	During	After
H2O2 - colourless solution	- bubbling alot	- white bubbles
liver discs - reddish paper	- water being displaced by gas	- very light brownish translucent solution with lighter red paper discs

 

Quantitative Observations

H2O2 (aq) used (mL)	[enzyme] (# discs)	O2 (g) produced (mL)
5.0	1	55.0
5.0	3	52.0
5.0	5	50.0
5.0	7	48.0